The following pages and posts are tagged with
| Title | Type | Excerpt |
|---|---|---|
| Single Cell RNAseq – encapsulation and library prep | Page | Using the 10X Chromium Controller to encapsulate individual cells for single cell genomic experiments |
| SMARTer Stranded Total RNA-Seq Kit v2- Pico Input Mammalian | Page | This protocol walks you through preparing a total transcriptome RNA library for RNAseq when your starting amount of RNA is less than ~50ng or if you are work... |
| Illumina Nextera Flex Sample Preparation Protocol | Page | Preparation of sequencing libraries from DNA or cDNA for sequencing on an Illumina platform |
| Illumina Nextera XT Sample Preparation Protocol | Page | Preparation of sequencing libraries from DNA or cDNA for sequencing on an Illumina platform |
| RNAseq FAQs | Page | information on our technology, how you gain access, how much experiments cost, and our 'hands-on' philosophy. |
| Project management and quality assurance for RNAseq projects | Page | Managing your RNAseq project and checking the quality of raw data are essential first steps in the analysis of any RNAseq experiment. The protocol below out... |
| Depositing RNAseq data to GEO | Page | This page will walk you through the steps to make your data publicly available so you can meet the requirements for manuscript submission and publication |
| Illumina TruSeq Stranded mRNA Sample Preparation Protocol | Page | Preparation of mRNA libraries for sequencing on an Illumina platform |
| Single Cell RNAseq – encapsulation | Page | Using droplet-based technology to isolate and barcode individual cells |
| Single Cell RNAseq – library prep | Page | post-encapsulation steps for preparing sequence-ready libraries for scRNAseq |
| Kallisto | Page | Kallisto is our first choice for aligning reads because it combines speed, accuracy, and the ability to leverage bootstraps for modeling technical variance |
| mRNA-seq library prep for low input samples | Page | This protocol walks you through preparing a mRNA library for RNAseq when your starting amount of RNA is less than ~75ng. This is a useful protocol if you ar... |
| scRNAseq FAQs | Page | information on our single cell RNAseq (scRNAseq) technology, how you gain access, and how to think about the the cost of a typical scRNAseq experiment. |
| Illumina TruSeq Stranded total Sample Preparation Protocol | Page | Preparation of total transcriptome libraries for sequencing on an Illumina platform |