Forward Primers for V4 region of 16S
v4.SA501	AATGATACGGCGACCACCGAGATCTACACATCGTACGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA502 	AATGATACGGCGACCACCGAGATCTACACACTATCTGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA503 	AATGATACGGCGACCACCGAGATCTACACTAGCGAGTTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA504 	AATGATACGGCGACCACCGAGATCTACACCTGCGTGTTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA505 	AATGATACGGCGACCACCGAGATCTACACTCATCGAGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA506 	AATGATACGGCGACCACCGAGATCTACACCGTGAGTGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA507 	AATGATACGGCGACCACCGAGATCTACACGGATATCTTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SA508 	AATGATACGGCGACCACCGAGATCTACACGACACCGTTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB501 	AATGATACGGCGACCACCGAGATCTACACCTACTATATATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB502 	AATGATACGGCGACCACCGAGATCTACACCGTTACTATATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB503 	AATGATACGGCGACCACCGAGATCTACACAGAGTCACTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB504 	AATGATACGGCGACCACCGAGATCTACACTACGAGACTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB505 	AATGATACGGCGACCACCGAGATCTACACACGTCTCGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB506 	AATGATACGGCGACCACCGAGATCTACACTCGACGAGTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB507 	AATGATACGGCGACCACCGAGATCTACACGATCGTGTTATGGTAATTGTGTGCCAGCMGCCGCGGTAA 
v4.SB508 	AATGATACGGCGACCACCGAGATCTACACGTCAGATATATGGTAATTGTGTGCCAGCMGCCGCGGTAA

Reverse Primers for V4 region of 16S
v4.SA701 	CAAGCAGAAGACGGCATACGAGATAACTCTCGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA702 	CAAGCAGAAGACGGCATACGAGATACTATGTCAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA703 	CAAGCAGAAGACGGCATACGAGATAGTAGCGTAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA704 	CAAGCAGAAGACGGCATACGAGATCAGTGAGTAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA705 	CAAGCAGAAGACGGCATACGAGATCGTACTCAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA706 	CAAGCAGAAGACGGCATACGAGATCTACGCAGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA707 	CAAGCAGAAGACGGCATACGAGATGGAGACTAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA708 	CAAGCAGAAGACGGCATACGAGATGTCGCTCGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA709 	CAAGCAGAAGACGGCATACGAGATGTCGTAGTAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA710 	CAAGCAGAAGACGGCATACGAGATTAGCAGACAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA711 	CAAGCAGAAGACGGCATACGAGATTCATAGACAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SA712 	CAAGCAGAAGACGGCATACGAGATTCGCTATAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB701 	CAAGCAGAAGACGGCATACGAGATAAGTCGAGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB702 	CAAGCAGAAGACGGCATACGAGATATACTTCGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB703 	CAAGCAGAAGACGGCATACGAGATAGCTGCTAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB704 	CAAGCAGAAGACGGCATACGAGATCATAGAGAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB705 	CAAGCAGAAGACGGCATACGAGATCGTAGATCAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB706 	CAAGCAGAAGACGGCATACGAGATCTCGTTACAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB707 	CAAGCAGAAGACGGCATACGAGATGCGCACGTAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB708 	CAAGCAGAAGACGGCATACGAGATGGTACTATAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB709 	CAAGCAGAAGACGGCATACGAGATGTATACGCAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB710 	CAAGCAGAAGACGGCATACGAGATTACGAGCAAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB711 	CAAGCAGAAGACGGCATACGAGATTCAGCGTTAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT 
v4.SB712 	CAAGCAGAAGACGGCATACGAGATTCGCTACGAGTCAGTCAGCCGGACTACHVGGGTWTCTAAT

Sequencing primers for V4 region
read1	TATGGTAATTGTGTGCCAGCMGCCGCGGTAA		
read2	AGTCAGTCAGCCGGACTACHVGGGTWTCTAAT
index	ATTAGAWACCCBDGTAGTCCGGCTGACTGACT